ABSTRACT
Objective To evaluate the therapeutic efficacy of a combination of proton pump inhibitor(PPI) and hydrotalcite for endoscopic submucosal dissection (ESD) induced ulcer.Methods Eightyone consecutive patients who underwent ESD were randomly assigned to either the group of PPI and hydrotalcite therapy ( drug combination group,n =41 ) or the PPI group ( n =40).Delayed bleeding rates were monitored and compared.The main upper abdomen symptoms 1-wk later and ulcer healing rates and ulcer diameter 4-wk later were compared between the two groups.Results There was no significant difference in delayed bleeding rate (P ＞ 0.05 ).At the end of first week after ESD,the combination therapy was significantly more effective than the PPI alone in reducing frequencies and severity of upper abdominal pain and upper abdominal distention,while there was no significant difference between the two groups in relieving belch and nausea.A better ulcer healing rates and a smaller ulcer diameter were observed in the combination group at the end of 4 weeks ( P ＜ 0.05 ).Conclusion The combination therapy of hydrotalcite and PPI can relieve upper abdominal symptoms and improve the healing rate of ESD induced ulcer.
ABSTRACT
A plane, three-dimensional chamber of pulsatile-flow-cultivation and the liquid store chamber connected by medical silica gel tube were designed and constructed by ourselves according to the design principle. The rotator pump of cardiopulmonary bypass unit was acted as the power source. The mixed gas containing 5% CO2 and 95% air was supplied through the ventilation orifice of the liquid store chamber. The temperature of these components was stabilized by thermostatic waterbath. The test of biomechanics and biological compatibility for the system was carried out by cultures experiment during two weeks. The results of the experiment showed that there was no leak in the pulsatile-flow-cultivation components in which the concentration of CO2 was controlled about 5%+1%, the temperature at 37 +/- 1 degree C, and the value of pH between 6.8 and 7.5. The flow rate of the system could be adjusted exactly between 0.125 L/min and 6.0 L/min. The endothelial cells on the viable homograft valve increased about 10 times after being cultured for 2 weeks. The cultures of cell and mould taken from the leaf and Dacron cloth of homograft valve were reported to be negative. The results of the experiment demonstrated that there was satisfactory homeostasis of these components in effective modeling pulsatile-flow-field for the implantation cells cultured, proliferated, and remodeled under the condition inferior or superior to physiological level in vitro. The system can meet the need for study of pulsatile-flow-cultivation and tissue engineering heart valve constructed in vitro.
Subject(s)
Humans , Biocompatible Materials , Chemistry , Biomechanical Phenomena , Cells, Cultured , Computer Simulation , Endothelial Cells , Cell Biology , Heart Valve Prosthesis , Models, Biological , Prosthesis Design , Pulsatile Flow , Physiology , Tissue Engineering , Methods , Tissue ScaffoldsABSTRACT
Objective Using a bioreactor to culture reendothelializing homograft bioprosthetic valve (HBV) in order to ultimately form viable tissue in vitro, so as to provide basic material for animal experiment and clinical application. Methods The reendothelializign HBV was placed in a pulse duplicator system (“bioreactor”), designed and fabricated by us. These grown under gradually increasing nutrient media flow and pressure for additional 14 days: 125 ml/min at 30 mm Hg (days 1-4), 250 ml/min at 40 mm?Hg (days 5-7), 500 ml/min at 50 mm Hg (days 8-10), 750 ml/min at 75 mm Hg (days 11 to 14). Throughout the studies, the flow device was placed in an incubator with 95% humidity and 5% carbon dioxide. The morphologic structure was observed and photographed by stereomicroscope with 0.5% silver nitrate (AgNO_3)staining and SEM on the 7th, 10th and 14th day. The retention rate of the ECs overlaid onto the HBV was evaluated with the percentage (%). Results The reendothelization level on the surface of the leaflets was 92% before pulsatile-flow-cultivation. But the rate of ECs retention on the scaffold exposured to pulsatile flow was only 36%, 23% and 11% on the 7th, 10th and 14th day, respectively. Conclusion The retention rate of ECs on the scaffold was low for pulsatile-flow-cultivation in vitro. Problems, including deficiency of mature stress fibers in the endothelial cells from MSCs deficiency of the adherence and growth factors still need improvement.